Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Lactate oxidation reaction. The reaction shows the oxidation of lactate to pyruvate by lactate oxidase (LOx) in the presence of oxygen (O 2 ), generating hydrogen peroxide (H 2 O 2 ) as a byproduct.

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques:

Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Review of printed sensors for enzymatic detection of the lactate analyte. NR: not reported. HRP: horseradish peroxidase

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques: Modification, Encapsulation, Membrane